Composite

Part:BBa_K3352006:Design

Designed by: Hannah Hsu   Group: iGEM20_TAS_Taipei   (2020-10-18)


T7 + RBS SplintR Ligase Expressing Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 835
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We attached a 6x histidine tag (6x His-Tag) upstream of the SplintR Ligase sequence for purification purposes followed by a glycine-serine linker (GS linker).



Source

We obtained the amino acid sequence of the SplintR ligase protein found in Paramecium bursaria Chlorella virus 1.

References